Ten years after the first American Pie movie, three new hapless virgins discover the Bible hidden in the school library at East Great Falls High. Unfortunately for them, the book is ruined, and with incomplete advice, the Bible leads them on a hilarious journey to lose their virginity.
| Directing | John Putch | Director |
| Writing | David H. Steinberg | Writer |
| Writing | Adam Herz | Characters |
| Production | Craig Perry | Executive Producer |
| Production | Warren Zide | Executive Producer |
| Production | Mike Elliott | Producer |
| Camera | Ross Berryman | Director of Photography |
| Production | Greg Holstein | Associate Producer |
| Production | Simon Abbott | Line Producer |
| Art | Tony Devenyi | Production Design |
| Art | Teresa Weston | Art Direction |
| Costume & Make-Up | Cynthia Ann Summers | Costume Design |
| Art | Jennifer Kom-Tong | Set Decoration |
| Editing | John Gilbert | Editor |
| Production | Jan Glaser | Casting |
| Production | Sean Cossey | Casting |
| Production | Stuart Aikins | Casting |
| Sound | Paul DiFranco | Music Supervisor |
| Sound | David Lawrence | Original Music Composer |
| Production | Simon Abbott | Unit Production Manager |
| Directing | Allan Harmon | First Assistant Director |
| Directing | Victor Landrie | Second Assistant Director |
| Crew | Ernie Jackson | Stunt Coordinator |
| Crew | Yves Cameron | Stunt Coordinator |
| Crew | Guy Bews | Stunt Coordinator |
| Crew | Bruce Crawford | Stunt Double |
| Crew | Brett Armstrong | Stunt Double |
| Crew | Noah Beggs | Stunt Double |
| Crew | Krista Bell | Stunt Double |
| Camera | Ryan McMaster | "A" Camera Operator |
| Camera | Richard Eagan | First Assistant "A" Camera |
| Camera | Mark Weinhaupl | Second Assistant "A" Camera |
| Camera | Lou Gruzelier | "B" Camera Operator |
| Camera | Lou Gruzelier | Steadicam Operator |
| Camera | Ivan Maldonado | "B" Camera Operator |
| Camera | Ivan Maldonado | Steadicam Operator |
| Camera | Jos Oman | First Assistant "B" Camera |
| Camera | Laura Hyvarinen | Second Assistant "B" Camera |
| Camera | Frederick Heartline | Camera Trainee |
| Camera | Chris Helcermanas-Benge | Still Photographer |
| Directing | Alexandra La Roche | Script Supervisor |
| Sound | Michael Williamson | Sound Mixer |
| Sound | Kristian Bailey | Sound Mixer |
| Sound | Rick Bold | Boom Operator |
| Sound | Chris Glyn-Jones | Boom Operator |
| Sound | Kristian Bailey | Sound Assistant |
| Sound | Chris Higgins | Sound Assistant |
| Art | Bobbi Allyn | Assistant Set Decoration |
| Costume & Make-Up | Courtney McKenzie | Assistant Costume Designer |
| Costume & Make-Up | Joshana McMahon | Key Costumer |
| Costume & Make-Up | Deb Schrieber | Costumer |
| Costume & Make-Up | Sabrina Kolyck | Costumer |
| Costume & Make-Up | Ines Zagoudakis | Costumer |
| Costume & Make-Up | Allison Chretien | Costumer |
| Costume & Make-Up | Wendy Levin | Costumer |
| Costume & Make-Up | Issa Mills | Costumer |
| Costume & Make-Up | Issa Mills | Costumer |
| Costume & Make-Up | Jennifer Kipps | Key Makeup Artist |
| Costume & Make-Up | Lyne Denomme | First Assistant Makeup Artist |
| Costume & Make-Up | Tanya Howard | Assistant Makeup Artist |
| Costume & Make-Up | Joel Echallier | Special Effects Makeup Artist |
| Costume & Make-Up | Robert A. Pandini | Hairstylist |
| Costume & Make-Up | Dean Scheck | First Assistant Hairstylist |
| Crew | Darren Marcoux | Special Effects Coordinator |
| Crew | Phil Davis | Special Effects Assistant |
| Production | Stacy A. Harris | Production Coordinator |
| Production | James Fantin | Assistant Production Coordinator |
| Directing | David Heimbecker | Third Assistant Director |
| Directing | Dezi Haddock | Assistant Director |
| Directing | Marijke Richman | Assistant Director |
| Production | Rebecca Zoe Leigh | Casting Associate |
| Production | J.J. Ogilvy | Casting Associate |
| Directing | Mike Elliott | Second Unit Director |
| Directing | Victor Landrie | Second Unit First Assistant Director |
| Camera | Danny Nowak | Second Unit Director of Photography |
| Camera | Paul Mitchnik | Second Unit Director of Photography |
| Camera | Danny Nowak | Camera Operator |
| Camera | Paul Mitchnik | Camera Operator |
| Camera | Toby Armitt | First Assistant Camera |
| Camera | Sacha Fassaert | First Assistant Camera |
| Camera | Chris Weiss | Second Assistant Camera |
| Camera | Mike Tanassee | Second Assistant Camera |
| Crew | Jan Kikumoto | Post Production Supervisor |
| Editing | James G. White | Assistant Editor |
| Crew | Colin Laurent-Bixler | Post Production Assistant |
| Crew | Eunice Choi | Post Production Assistant |
| Sound | Patrick Giraudi | Supervising Sound Editor |
| Sound | Patrick Giraudi | Sound Re-Recording Mixer |
| Sound | Kelly Vandever | Sound Re-Recording Mixer |
| Sound | Steven Avila | Sound Effects Editor |
| Sound | Peter D. Lago | Sound Effects Editor |
| Sound | Alexander Pugh | Sound Effects Editor |
| Sound | Patrick Giraudi | Dialogue Editor |
| Sound | Brian S.M. Wroth | ADR Editor |
| Sound | Ben Whitver | ADR Mixer |
| Sound | Doug Latislaw | ADR Mixer |
| Sound | Irene Montero | Foley Editor |
| Sound | Greg Mauer | Foley Mixer |
| Sound | Adam DeCoster | Foley Artist |
| Sound | Rick Owens | Foley Artist |
| Sound | Sean Madsen | Sound Engineer |
| Sound | Steven Utt | Sound Engineer |
| Sound | Gordon Sproule | ADR Mixer |
| Sound | Amy Giammarco | ADR Coordinator |
| Sound | Adrian van Velsen | Music Editor |
| Sound | Jean-Paul DiFranco | Music Coordinator |
| Visual Effects | Ron Simonson | Visual Effects Supervisor |
| Visual Effects | Chris Bremble | Visual Effects Producer |
